Journal: Materials Today Bio

Article Title: Injectable anti-inflammatory, antioxidant supramolecular nanofiber hydrogel for peripheral nerve injury repair and neuropathic pain relief

doi: 10.1016/j.mtbio.2026.102780

Figure Lengend Snippet: Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), Gap43 immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: The antibodies used and their dilution ratio include: NF-κB (CST, 1:1000), P- NF-κB (CST, 1:1000), Gap43 (CST, 1:1000), S100β (Abcam, 1:1000), GAPDH (Proteintech, 1:1000).

Techniques: Staining, Immunohistochemistry, Western Blot, Expressing, Software

Journal: Investigative ophthalmology & visual science

Article Title: Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules.

doi: 10.1167/iovs.19-27566

Figure Lengend Snippet: FIGURE 4. Interaction between lenticule neurites and SCs. Colocaliza- tion of SC marker expression and stromal neurites by confocal immunofluorescence: (A–C) GAP43 (green) and TuJ1 (red). (D–F) p75NTR (green) and TuJ1 (red).

Article Snippet: Antibodies Used in This Study Antibody Source Working Concentration 1 bIII-tubulin Covance MMS435P 0.5 lg/mL 2 GAP43 Novus NB300-143 0.5 lg/mL 3 P75NTR Millipore AB1554 0.5 lg/mL 4 Phalloidin-AlexaFluor 594 conjugate Invitrogen 0.5 lg/mL 5 AlexaFluor 488 goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 6 RedX-conjugated goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 Downloaded from iovs.arvojournals.org on 10/24/2019 placed as flat-mount.

Techniques: Marker, Expressing

Journal: Journal of Neuroscience

Article Title: Toll-Like Receptor Signaling Is Critical for Wallerian Degeneration and Functional Recovery after Peripheral Nerve Injury

doi: 10.1523/jneurosci.3027-07.2007

Figure Lengend Snippet: Figure 3. Deficiency in TLR signaling compromises macrophage recruitment/activation and delays myelin debris clearance, axonal regeneration, and locomotor recovery after sciatic nerve lesion. A–D, Representative fluorescence photomicrographs takenfromlongitudinalsectionsofthesciaticnervedistaltothelesionshowingCD68-immunopositivemacrophagesinC57BL/6J (A),TLR2-ko(B),C3H/HeOUJ(C),andTLR4d(D)miceat7d.E,QuantificationofthenumberofCD68macrophagesinthesciaticnerve distalstumpofTLR2-ko,TLR4d,MyD88-ko,andwild-typemiceat7dafterlesion(n8pergroup).F–I,Bright-fieldphotomicrographs showingmyelinstainedwithLFBinthesciaticnervedistalstumpofC57BL/6J(F),TLR2-ko(G),C3H/HeOUJ(H),andTLR4d(I)miceat7d. J,QuantificationofLFBstainingofmyelininthedegeneratingsciaticnervedistalstumpofmicedeficientinTLRsignalingat7d(n8per group). K, Quantification of the number of axons that had regenerated up to 4 mm distal to the site of lesion, as visualized by GAP-43 immunofluorescence, at 4 d after lesion. L, M, Recovery of locomotor functions from a sciatic nerve lesion, as determined by the SFI, in TLR2-ko,MyD88-ko,andTLR4dmicecomparedwiththeirrespectivecontrolgroups(WT)overaperiodof49d(n12pergroup).***p 0.001,**p0.01,and*p0.05comparedwiththecontrolgroup.Scalebar,50m.

Article Snippet: Immunohistochemistry was performed to detect the following antigens: (1) mouse CD68 using the monoclonal anti-CD68 antibody (to visualize activated macrophages/monocytes; Serotec, Raleigh, NC), (2) growth-associated protein-43 (GAP-43) using the polyclonal antiGAP-43 antibody (to visualize regenerating axons; Novus Biologicals, Littleton, CO), (3) rat CD68 using the monoclonal ED-1 antibody (to visualize activated macrophages/monocytes; Serotec), (4) ionized calcium-binding adaptor molecule 1 (iba1) using the polyclonal antiiba1 antibody (to visualize macrophages/monocytes; Wako Chemicals USA, Richmond, VA), and (5) mouse CD45 using the monoclonal antiCD45 antibody (to visualize leukocytes; BD PharMingen, Mississauga, Ontario, Canada).

Techniques: Activation Assay, Fluorescence, Immunofluorescence